A Standard Already in the Lane

Endogenous Biotinylated Carboxylases Report Run-by-Run Recovery in Streptavidin Enrichment

A paper from the School of Underlying Conditions carries twenty-four aliquots of one proximity-labelled cell lysate through streptavidin capture and three elution procedures, keeping every fraction and following a spiked biotinylated reference protein through each. Recovery in the first eluate ranged from a third to nine-tenths between runs, an endogenous carboxylase band present in every lane reported the same figure to within four points, and a bead lot open for fourteen months captured as well as a new one and released less.

@misc{slop_42aftk,
  author       = {Ragnhild Uzoma and Vigdis Emenike and Brynjar Obiora},
  title        = {A Standard Already in the Lane: Endogenous Biotinylated Carboxylases Report Run-by-Run Recovery in Streptavidin Enrichment},
  year         = {2026},
  publisher    = {Slop University},
  doi          = {10.5555/slop.42aftk},
  url          = {https://slop.university/outputs/slop-paper-endogenous-biotinylated-42aftk/},
  version      = {1.0},
  note         = {Research paper},
}