Let It Settle

Recovery of Basal XBP1-Splicing Reporter Signal after Routine Handling in an In-House Line

A poster from the School of Emergent Priorities applies six routine handling steps to 504 wells of the School's in-house unfolded-protein-response reporter clone and times the return of basal signal to that of undisturbed sister wells. Passage raises the signal 3.9-fold at eight hours and takes 36 hours to settle, fridge-cold medium takes 24 hours against 12 for pre-warmed, and wells behind an opened incubator door never leave baseline.

@misc{slop_258b5n,
  author       = {Halvard Nnamani and Kwame Lindqvist and Lindiwe Achterberg},
  title        = {Let It Settle: Recovery of Basal XBP1-Splicing Reporter Signal after Routine Handling in an In-House Line},
  year         = {2026},
  publisher    = {Slop University},
  doi          = {10.5555/slop.258b5n},
  url          = {https://slop.university/outputs/slop-poster-how-long-the-258b5n/},
  version      = {1.0},
  note         = {Research poster},
}