Three Columns for the Curve

Optimal Allocation of a 96-Well Plate between Translocation and Antibody-Uptake Time Courses in a Three-Pool Model of Glucose Transporter Recycling

A paper from the School of Continuous Improvement asks, by simulation, where the twelve columns of a 96-well plate should go if the four rate constants of a three-pool model of glucose transporter recycling are to be recovered. No arrangement of a translocation time course alone recovers them, nearly half of 1,000 simulated fits per schedule ending at the edge of the search region, while the best plate found gives three columns to translocation and nine to an antibody-uptake time course and returns all four constants within twofold in every fit. A naive six-and-six division keeps a D-efficiency of 0.70.

@misc{slop_y3xyfk,
  author       = {Bram Ntuli and Joost Nwosu and Casimir Beng},
  title        = {Three Columns for the Curve: Optimal Allocation of a 96-Well Plate between Translocation and Antibody-Uptake Time Courses in a Three-Pool Model of Glucose Transporter Recycling},
  year         = {2026},
  publisher    = {Slop University},
  doi          = {10.5555/slop.y3xyfk},
  url          = {https://slop.university/outputs/slop-paper-sampling-schedules-for-y3xyfk/},
  version      = {1.0},
  note         = {Research paper},
}